Hello everyone,
I'm starting working with SW48 colon cancer cells in vivo, and I wondered if Matrigel is needed for a subcutaneous injection. And, how much cells do you generally inject?
Thanks a lot for your help!!!
Hello
Yes you can do that . in general we used subcutaneous Injection used to significantly enhanced metastatic dissemination.
see these links
http://www.jove.com/visualize/abstract/24487410/subcutaneous-preconditioning-increases-invasion-metastatic
http://www.bio-protocol.org/e166
hello,
I had also worked on B16F10 melanoma cancer cell line in C57BL mice. So i had Injected 5 Lakh cell/ 200ul with the help of 1ml syringe.I had counted cell in heamtocytometer dye with typhen blue dye.May be its helpful for you.
SW480 cell form tumors upon the injection i.p. of 2x10(6) cells within 2 weeks. If you plan the s.c. administration, 0.5-1.0x10(6) cells should be enough. You don't need the Matrigel, albeit it keeps the cells together and thus supports the tumor development. If you will use the Matrigel, the number of cells injected can be even lower.
- Badges
- Science topic
- Similar topics
- Biological Science
- Cell Biology