Who is the best provider of agarose (quality vs price) for analysis of PCR products?

More François W. Paradis's questions See All

Is Analysis of Similarity (ANOSIM) sensitive to differences in sample size?

Hi there. I want to compare 10 small forest landscapes on which different harvest designs were applied and see how much their attributes (structure, species, soil moisture...) differ. ANOSIM seems...

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Is there a way to download all molecules from a published research paper?

Hello, Is there a (preferably free) way to download all molecules (SMILES or SDF) from a published scientific article? Like searching pubchem or chembl or ZINC via DOI or so? I would like to...

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How to add a journal ?

Hello, is it possible to add a journal ? (Les Cahiers de Neuropsychologie Clinique) Some of my references can't be complete. Thank you.

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What would be an appropriate method to compute the watershed of a lake?

Hi there, I'm wondering what would be the best approach to create the watershed of a lake. I see two solutions here: - Creating the watersheds of all tributaries of the lake and aggregate them,...

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Is surface water supply a hydrologic service, a forest service, or a watershed service?

There is no doubt that the access to surface freshwater is an ecosystem service. However, I feel that the many publications on ecosystem services and disservices with respect to the water supply...

07 January 2019 4,166 13 View

What are your tips for obtaining maximum resolution on an agarose gel?

I want to resolve very small deletions on agarose gel. My fragments are ~ 200 bp. What are your tips to get the best possible resolution? Here is what I have already gathered around: - 4%...

18 November 2018 5,740 6 View

What is your favourite long-range PCR enzyme?

I want to amplify a long region of around 20 kb from human genomic DNA. I understand this is around the maximum of what is feasible, but looks possible, at least according to manufacturers'...

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I am looking for post-doctoral position that may begin in the fall. Can you help?

I defended my dissertation for the Payson Program for Global Development at Tulane University in May and I am currently working on the required edits. The title of my dissertation is "A...

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How to install Neo (Python package for working with electrophysiology) for MAC?

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Anyone using Brookhaven BIC90 for zeta potential measurements. What is the polymer of the Organic Solvent Electrode?

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Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

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What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

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How do I increase the yield of my PCR product?

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SDS page software for analysis?

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

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Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

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Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

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Nadine A. Gund

Low-melting agarose, "standard" agarose,...?

François W. Paradis

Standard

Try the agrose from "Roth" (--> Neeo Quality) . The Low melting agarose and other special agarose are from "Biozym".

All the best, Nadine

Serdar dinç

Agorose from Sigma is also very good for such studies...

Roger S Rowlett

We buy whatever is cheapest. It all melts fine in the chaotropic reagents used in the gel purification kits.

Haitham E. M. Zaki

This actually depends on the purpose of the PCR. Is it to study the expression? or for example to check the size of the bands like in SSR.