How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

I teach a STEM career course for IT majors at a small liberal arts school. I am looking for collaborators/information sources for a new book ?

Looking for collaborators/info sources for a new book I am writing in the career/life coaching field on "Adult Bullies". Most research has been done on childhood bullying, however they eventually...

27 November 2020 6,532 1 View

What solvent can I use to prepare a higher concentration of 5-Bromouracil?

I am standardizing a UHPLC method for separation of thioguanine phosphate metabolites. For this, I want to use 5-Bromouracil (5-BU) as an internal standard, however I am unable to prepare solution...

15 November 2020 4,982 3 View

Is annealing should be done again after a year of nano-particles fabrication and they are found to be agglomerated?

I have prepared CeO2 based nano-particles. due to some technical issues some of the properties like electronic structure and magnetic properties are pending. the samples were prepared...

08 October 2020 769 3 View

Advice writing an unbiased qualitative research question?

Hi, I was wondering whether kindly souls out there might be able to help me with some advice regarding the formulation of a RQ for my qualitative study please? I'm trying to refine an early...

25 March 2020 6,512 5 View

Is there any python code which converts text(unstandarized) into a csv fom?

I have an unstandarized text data . So when I convert the text into csv form it results are not into a proper csv format.

05 February 2020 4,556 3 View

Do you think the word/phrase "Economic Growth" is an innocent and positive one?

15 January 2020 2,890 14 View

What ML/AI algorithms can I use to analyze Bank statement and detect frauds??

Ml algorithms which can be useful to analyze bank statement and then extract information about that frauds.

08 January 2020 9,890 6 View

Any free software to convert my audio interview to text ?

I am doing Qualitative Research

31 December 2018 4,092 8 View

What are barnacles environmental constraints ?

I've got some data on barnacles across a shore line and was after a guide to the appropriate review that discuses the biochemical changes within barnacles during desiccation. Is there one that...

03 December 2018 7,280 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View