Hi everyone. I'm in a big confusing dilema. I have the idea to develop a differential hosts set for downy mildew races identification in quinoa. However, there's no information (or at least i couldn't find it) about P. variabilis races neither quinoa major resistence genes in commercial varieties. So i start searching for differential hosts development in another crops. I just could find the applications of already existing sets to pathogen races already identified, for example, for spinach, tomato, cucumber, coffea, etc. So, I'd like to know, What's the first step to study the genetic interaction of a poorly studied pathogen in a poorly study crop, the identification of pathogen races or resistence varieties? Do you know examples of another crops? Thanks, excuse me for my bad english and happy holidays!
Dear Héctor Andrés Ramírez
Its depend on the project goals
DHS are sets of plant varieties used to define strains of plant pathogens based on susceptible and resistant reactions. Reference pathogen strains are known, characterized isolates of a given pathogen.
A DHS is defined by varieties from within one or several plant species that are hosts to the pathogen. For example, races of Bremia lactucae causing downy mildew in lettuce are determined solely by using lettuce cultivars. But in the case of root knot caused by Meloidogyne spp. the set is made up of several host species - tobacco, cotton, watermelon, tomato, pepper and peanut. The hosts utilized within differential sets are often existing varieties or experimental species known as indicator hosts.
Having these identified and available as a “set”, while not a high volume need, has extensive value as is apparent in looking at the cost of independently identifying the material and gathering them into a “known state” for use in testing.
Pls. find the attached files
Page 7 in: Danielsen, S., Ames, T.. 2004. Mildew (Peronospora farinosa) of quinua (Chenopodium quinoa) in the Andean region: Practical manual for the study of the disease and pathogen International Potato Center, Pp.30
Page 36 in: Jose Benjamin Ochoa Lozano. Pernospora Farinosa, F. Mp. Chenopodii. in Quinoa Aspecte of Epidemiology and Resistance INIAP Archivo Historico
http://onlinelibrary.wiley.com/doi/10.1046/j.1365-3059.1999.00352.x/full
https://www.researchgate.net/publication/40138798_Postulation_of_virulence_groups_and_resistance_factors_in_the_quinoadowny_mildew_pathosystem_using_material_from_Ecuador
http://www.seedcentral.org/documents/ASTA_CPPSI_Differential_Host_Sets_Short_Summary.pdf
http://www.worldseed.org/our-work/plant-health/differential-hosts/
http://www.worldseed.org/our-work/plant-health/plant-disease-and-resistance/
http://192.156.137.121:8080/cipotato/publications/pdf/003681.pdf
https://www.researchgate.net/publication/262269612_Multiplex_real-time_PCR_assays_for_detection_of_four_seedborne_spinach_pathogens
http://worldwidescience.org/topicpages/m/mildew+peronospora+belbahrii.html
http://www.mdpi.com/1422-0067/14/5/8985/htm
Hoping this will be helpful
Regards
Prof. Houda Kawas
Article Postulation of virulence groups and resistance factors in th...
Article Multiplex real-time PCR assays for detection of four seedbor...
Dear , Héctor Andrés Ramírez.
You can also depend on the attached article .
With my best wishes
Yehya. I already read that paper, but I don't know if the material they are assessing (refered as lines) are genetically homogeneous. Many quinoa cultivar came from mass selection breeding, so them could carry more than one resistence factor. Thanks for your response.
Prof, Houda Kawas, Thanks for your explanation and for the papers (it will take me some time to read all but I will). One more question (if it isn't answer on the articles) How to identify those "Reference pathogen strains" before developing a DHS?
You most welcome
you may find all the answers after reading the articles
Houda
139
Hi Héctor
I think, you can identify the pathogen strains by doing the pathogenicity tests
- Badges
- Science topic
- Similar topics
- Biological Science
- Genetics