in my research, I would like to edit one of the gene related to obesity in vivo and I want to find out a successful database that could accurately measure the percentage of on-targeitng?
Benchling
CasOT , crispr P, offscan, crista etc
is benchling paid or free?
When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...
03 March 2021 4,892 5 View
Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...
03 March 2021 6,317 1 View
Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...
03 March 2021 6,193 1 View
We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...
01 March 2021 1,899 2 View
Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...
28 February 2021 8,189 3 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
I have a pipe with 10 mm in Diameter and 2 bar pressure, the fluid velocity is 200 m/s, the fluid is air. is there a way to reduce velocity without affecting the pressure value?
25 February 2021 7,187 3 View
24 February 2021 5,378 1 View
In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...
21 February 2021 1,864 2 View
I am currently doing my Master Thesis in FinTech and I am thinking of doing my project on the impact of AI and ML on the financial industry but as the impact of these technologies on the industry...
21 February 2021 7,128 3 View
Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...
27 February 2021 1,965 1 View
I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...
22 February 2021 4,374 3 View
There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...
18 February 2021 9,109 5 View
Hello, I am currently using the Malvern Zetasizer software for the purpose of obtaining the size distribution of nanoparticles produced in the lab. When I perform the runs in the software to get...
15 February 2021 4,251 1 View
The goal of regression model is either to minimize the prediction error or to maximize a classification accuracy depending on the type of the model. Is there any optimization algorithm that is...
10 February 2021 4,128 3 View
When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...
09 February 2021 5,423 1 View
Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...
08 February 2021 2,727 5 View
I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...
08 February 2021 2,032 2 View
I am working on gait analysis using a feed-forward NN. When I use data from the accelerometer (3 columns for each axis if stored in a data frame), I get an accuracy of 95%, and when I used data...
07 February 2021 9,654 6 View
Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...
06 February 2021 2,847 2 View