For example, we have 100 samples, among them 50 are resistant, others are susceptible. Traditionally we sequenced 100 samples one by one ,and then call SNPS and other analyses. I wonder whether is it ok to seperate the 50 resistant samples into 10 groups for example, and then sequenced the groups as a total. If so ,we just need to sequence 10 groups which is cost effective and i think this will not influence too much for subsequent analysis. Anyone have published similar ideas in paper or have discussed it?
Maybe for humans,we need more accurate and more accurate. However for practical uses, we need to banlance. Especially, for a large samples, such as 50000 or more?
I think it is not correct to sequence a group of samples altogether. This will finally end up with unspecific results or results will need highly sophisticated SNP data analysis. I would still use the traditional approach you mentioned
Hello Jie
in this case there is a practical approach . we always use SSCP before sequencing . in this approach you will need to sequence only 3-5% of your samples with totally accepted accurate .
it is simple and cost effective technique
mail me for more details
regards
Hi Jie,
Multiplexing approach using bar-coding system makes it possible to group and sequence together many samples. In a case-control study, you could multiplex all cases together and all controls together.
Below, is a link where you could find more details on the multiplexing technology.
http://www.illumina.com/technology/next-generation-sequencing/multiplexing-sequencing-assay.html
Best regards
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