Which yeast expression system should I use for the expression of cellulase gene..?

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

How to enrich pig excreta for increasing nutrient quality organically ?

Pig slurry is rich in major and minor nutrients. Is there any way to improve / Enrich its manure quality to be used in agriculture organically ? please share your knowledge.

09 August 2024 5,605 2 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Leaf area of tomato ?

Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?

06 August 2024 2,508 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to preform densitometry on SDS-page bands?

I ran a SDS-page of a bacterial lysate and I want to quantify protein concentration in a specific band. I was thinking of using a standards ladder or make some standards are different...

05 August 2024 9,805 3 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

What are the limitations and challenges of using machine learning for predicting concrete compressive strength in practical applications?

Machine learning (ML) has shown great potential in predicting the compressive strength of concrete, an important property for structural engineering. However, its practical application comes with...

03 August 2024 2,546 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How do I convert cellulase enzyme 10,000U/g to mg/g fresh forage or mg/kg fresh forage?

I would like to know the minimum amount of Cellulase enzyme that could be used for silage making.

03 August 2024 3,170 1 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

10 July 2024 3,050 5 View

Is there any way to calculate whole genome similarity of organism without the whole genome sequencing ?

is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?

05 July 2024 4,070 3 View

Have pathomorphological studies of the lungs been performed in patients with desminopathy?

It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?

15 June 2024 1,355 7 View

Can yeast transformation not be done under a safety hood?

Hi All, This may sound like a stupid question, but I have a yeast transformation protocol that I am trying to modify for automation. Unfortunately, our automated equipment is not under any sort...

11 June 2024 5,146 2 View

How can I decide the variant I found segregating with a genetic condition is novel or known?

I found a variant segregating with a genetic condition, this variant is listed in ClinVar (with no clinical significance), gnomAD, etc. However, I could not find any publication reporting the...

10 June 2024 7,606 3 View

Describe the principles of Population Genetics and evolution as applied to livestock breeding programs?

Describe the principles of Population Genetics and evolution as applied to livestock breeding programs

08 June 2024 2,924 0 View

What is the suitable concentration of hygromycin in tomato sp. Micro-tom genetic transformation?

I used hygromycin and kanamycin for selecting positive buds in Micro-tom genetic transformation. Kanamycin performed very well and I could get positive plants soon, but hygromycin is a little bit...

29 May 2024 6,911 0 View

Ancy Thomas

HI, Pichia pastoris based expression system (Life Technologies) is widely been used for enzyme production. you read further and choose a suitable vector and compatible host for your purpose.

Rajesh K P

Pichia

Roger Palou

Pichia is really good and will give really big amounts of your protein. However, you should take into account that its big cell wall is something that can be problematic if you do not have the right equipment.

Yogesh Chaudhari

Thank you all...