By best, I mean which could easily extract out soil organic matter such as amino acids plus which could easily be available or synthesised using basic chemicals.
Literatures show that acetylacetone could be one of the best organic solvents to extract non-metalic element. This can be used by varying the solvent-soil sample ratio at pH 8.
Hey Segun Michael! First of all thanks a lot...
Actually I wanted to know about the solvent which can extract organic matter and not just non metallic elements, Under non metallic elements, inorganic compounds are also there right?
A new soil extractant (H3A) with the ability to extract NH4, NO3, and P from soil developed and tested against 32 soils, which varied greatly in clay content, organic carbon (C), and soil pH. The extractant (H3A) eliminates the need for separate phosphorus (P) extractants for acid and calcareous soils and maintains the extract pH, on average, within one unit of the soil pH. The extractant is composed of organic root exudates, lithium citrate, and two synthetic chelators (DTPA, EDTA). The new soil extractant was tested against Mehlich 3, Olsen, and water for extractable P, and 1 M KCl and water‐extractable NH4 and NO2/NO3. The pH of the extractant after adding soil, shaking, and filtration measures for each soil sample.
1. Morgan Extraction Reagent which was introduced in 1941
2. Mehlich introduced an universal extractant, frequently referred to as the Double Acid extractant, for assaying acid sandy textured soils.
3. The ammonium bicarbonate‐DTPA extraction reagent for use on alkaline soils was introduced.
4. Wolf modified the Morgan extraction reagent by adding DTPA to it. In 1984, Mehlich described his Mehlich No. 3 extraction reagent for use on a wide range of acid soil types.
All above extraction reagents can be assayed using a multielement analyzer such as the inductively coupled plasma emission specterometer for both the major elements and micronutrients in the obtained extractant.
The soil extract can be prepared by mixing 5g of soil in 1L H2O mix well, filter and then autoclave.You don't have to use soil extract to isolate Actinomycetes you can use any other medium that contained two different antibiotics for inhibition of fungi such as nystatin and streptomycin for inhibition of bacteria.
Dear Dr. Shivam Sahil , you have raised and interesting question.
According to Xian-you et al ( 2013 ) the use of 0.5 mol L-1 K2SO4 with a 5 times extraction was a better method to assess the concentration of ex tractable amino acids in soils.
Please go through the attached interesting article for the detail of the methodologies and findings.
https://ac.els-cdn.com/S2095311913602919/1-s2.0-S2095311913602919-main.pdf?_tid=c91f2390-f48e-42a6-90ac-264fd42e9469&acdnat=1524061923_8757bb156bea95a532be4a432d6ed49e
Dear Dr. Shivam Sahil , as an alternative to alcohol, Ba(OH)2 was found to be a promising extractant and proved to be very effective in recovering amino acids(PAUL AND SCHMID, 1960).
this link is useful
www.highplainsnotill.com/docs/Haney_et_al2006.pdf
regards
Follow this link of article
Article Humic materials from an organic soil: A comparison of extrac...
Best regards
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