What is the microscopic zoom requirement for observing bacterial movement under hanging drop?

More Shivam Sahil's questions See All

What is the fastest technique for soil bacteria identification?

I wanted to perform a quick experiment (10-25 minutes) which will help me to check microbial presence in soil, I have found many culture techniques but those at least take 8 hours of duration, I...

03 April 2018 6,160 10 View

Which would be best organic soil extractant?

By best, I mean which could easily extract out soil organic matter such as amino acids plus which could easily be available or synthesised using basic chemicals.

03 April 2018 4,107 8 View

How to access HSV based segmentation using matlab?

I tried to segment a rocks out of soil image using MATLAB, please help me with particular steps or guide me with more appropriate way. I tried using rgb segmentation to segment rock parts and I...

02 March 2018 6,982 7 View

Which sensor is best for monitoring soil temperature and compatible with micro controller such as arduino?

I have come across a lot sensors such as DHT-11/22, different types of temperature sensors, I need the most appropriate sensor for monitoring soil temperature. thanks!

31 December 2017 4,419 3 View

What should be pixel and resolution of a camera?

What should be appropriate pixel and resolution of a camera for digital image processing for soil analysis. how much zoom etc. would be most appropriate in elemental analysis.

10 November 2017 4,757 0 View

Can a rough soil sample be observed under a petrographic microscope?

How much X zoom would be perfect to observe a soil sample under petrographic microscope for further image processing?

10 November 2017 9,665 0 View

Is a pentographic microscope good for Digital image processing?

Is pentographic microscope good and accurate choice for elemental analysis of soil using digital image processing ? Can I get accurate readings for the soil analysis?

10 November 2017 9,187 3 View

Which wavelength light would be most appropriate to produce scattering in soil?

I want elemental analysis of soil using scattering properties, I am confused how to start with, in short I will be using a laser of certain wavelength, which is appropriate for observing in...

09 October 2017 9,300 0 View

What should be the intensity range for proper UV spectroscopy?

Hello there! I am working with a spectrometer project, where I would be using UV laser as light source, I want to use a cheap light detector and not photo multiplier tubes, I want to perform...

09 October 2017 3,632 0 View

Which is the better choice for soil analysis?

From basic texture and molecular orientations, to organic and in organic compositions, for proper analysis which spectrometer would be preferred for in field as well as in laboratory tests.

08 September 2017 7,904 6 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View