Almudena Ines Santos

5 Questions 6 Answers 0 Followers

Questions related from Almudena Ines Santos

Cerebral organoids, how to fix them for paraffin embedding?

I have been growing cerebral organoids from an iPSC line and currently need a fixation and paraffin embedding protocol for them. I have looked into several papers but can´t seem to find a suitable...

30 May 2023 6,174 2 View

Which would be a good combination of antibodies to detect Memory t cells by Immunofluorescence or Multiplexing?

I have been looking into specific markers to detect memory T cells, as there is not just one unique marker to detect them, I was wondering which would be a good combination, would CD45RO/CD3 work?

25 July 2018 1,678 5 View

Multiplex, on mouse tissues. Secondary antibodies

I have been trying the multiplexing protocol, for some trials on mouse tissues samples, but I still struggle with the secondary antibodies. Which ones would you recommend?

09 June 2017 5,633 1 View

Does someone have an H&E protocol for frozen OCT samples?

I need to do some H&E staining on OCT samples, I have done some before on paraffin sections, so if possible to do, I guess it should be similar. Thank you

26 January 2017 4,781 6 View

Can you use AEC and DAB for the same section in IHC?

I want to do a double staining on some IHC samples, can I use 2 different Peroxidase detection systems as AEC and DAB together? Which mounting media should I use (if the staining is possible)?

30 November 2016 5,384 4 View