Does anyone have experience using High Molecular Weight DNA Extraction Kits for extracting DNA from plant leaves?

Hi there, I'm looking for a High Molecular Plant DNA Extraction Kit for subsequent Nanopore sequencing. Does anyone have experience using commercially available kits? Any...

03 January 2021 9,487 4 View

How can I represent contact with cohesive behavior and friction in abaqus?

Hi, I am trying to represent an experimental test of pull out in abaqus, I have managed to get the detachment behavior (first section of the graph) but the part where the fiber slides does not...

30 November 2020 6,474 3 View

What is the best way to extract Spike protein from whole cell lysates for ELISA ?

Hello wise scientific community, I am trying to measure the concentration of Spike Protein ( whole protein) expressed by my cell line transfected with a pC DNA 3.1. I can see a nice band by...

17 September 2020 7,265 4 View

Does anyone know about public databases of baseline corticosterone in reptiles?

I'm searching some public databases of baseline corticosterone in reptiles, I know there is a public database of hormones by Vitousek et al. 2018 ( https://hormonebase.org/ ) and has some data...

31 July 2020 540 3 View

IEEE 34 node test feeder in DigSilent PowerFactory?

18 April 2020 1,949 2 View

How to associate parameters to function switch?

I am using comsol to calculate the IV curve using a physical model. The problem is that I am using the switch function to evaluate multiple spectra but I can not associate a temperature for each...

30 June 2019 2,894 1 View

Why in the simulation of a fracture toughness test, the values of stress intensity factor are not uniform along the crack of the specimen?

In the virtual simulation of toughness using a standard steel specimen (A516-Gr.70), the maximum equivalent (Von-Mises) stresses are uniform along the entire length of the crack. However, when the...

21 January 2019 3,694 1 View

Does anyone know an alternative co-stimulation signal to feeder cells or soluble CD28 for CD4 T cells?

I want to evaluate the CD28 loss on CD4 T cells in culture with different stimuli. I used to stimulate this cells with anti CD3/CD28 dynabeads but actually i don't know if it could interfere with...

31 December 2018 2,629 3 View

Can someone tell me where can I find the theory of anxiety postulated by Zung or the original manual?

I need it to write my thesis on anxiety and maternal attitudes in mothers in prision.

11 September 2018 9,452 1 View

How to design qPCR primers specific to cDNA in two different species (mouse & humans)?

Neither my supervisor or I have done qPCR for a number of years and he asked me to look into designing qPCR primers for detecting UCP1 expression in both humans and mice. We would prefer to use a...

10 September 2018 7,542 3 View

Could anyone recommend papers that focus on mixed-ethnicity Black-Caribbean/Caucasian adopted children?

Dear All, I have been looking for papers, or books, that focus on the issues surrounding adoption of mixed Black-Caribbean/Caucasian children. I am interested in literature that looks at any...

22 February 2021 8,466 2 View

Can you realize "top-down" and "bottom-up" ARE THE SAME THINGS at important junctures IN ONTOGENY (child development)?

Can you realize "top-down" and "bottom-up" ARE [ or certainly can, if not MUST, be ] THE SAME THINGS at important junctures IN ONTOGENY (child development)? This Question is NOT addressing YOU...

09 February 2021 5,289 1 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

Why sometimes current- voltage plot in chemiresistors/ FET does not pass through the origin?

I am trying to measure resistance from I-V curve from a chemiresistor/ FET sensor with a keithly sourcemeter. The substrate is soaked/ wet with protein and phosphate buffer only. But the I-V line...

24 January 2021 8,009 3 View

How can I verify the design on the FPGA ZedBoard?

I have designed AXI Interconnect to connect 4 masters and 1 slave using SystemVerilog and Vivado Tool. The simulation stage of the design is working well. In the design, there are more I/O pins...

06 January 2021 8,487 2 View

Transfecting high molecular weight poly I:C using lipofectamine 2000?

I'm planning to transfect HepG2 and Huh7 with poly I:C (high molecular weight poly IC from Invivogen using lipofectamine 2000 as transfectant reagent. I would like to do a standard curve using...

01 January 2021 239 1 View

What are the simplified method of extraction of alkaloid from a plant extract?

Descriptions and simple method of alkaloid extraction

01 January 2021 5,830 6 View

How to convert Terapeutic Human Doses to Cell Experimentation Doses?

"Toxicological evaluations revealed that Cur is found to be pharmacologically safe, even up to 12 g per day, as reported by several animal studies and in phase-I clinical trials Similarly,...

07 December 2020 3,012 1 View

Any advice on how to get KO cell lines by santa cruz Crispr-cas9 please?

Hello, I tried several times to obtain two KO cell lines for ERN1 (IRE1 alpha) using a santa cruz crispr cas9 "double nickase". (HNSCC cancer cell lines : PECA-PJ41 and PECA-PJ34). I tried...

07 December 2020 8,141 1 View

How to plot a rectification index curve (I/V plot)?

HEK cell is Clamped at different membrane potentials (-100 mV to +90 mV; 10 mv Step with Vh -60 mV) of GluK2 receptor and recorded the current by application of 10 mM L-Glutamate. Herewith I...

07 December 2020 2,002 1 View