Two good options for serological diagnosis of Q fever are ELISA and IFA. Which one is more reliable and convenient? Is there any species (Human,Cattle,Goat etc) specificity for diagnostic procedure?
I used fluorescein labeled C.burnetii for diagnosis of Q fever in human and animal. IFA is reliable and convenient for serological diagnosis of Q fever and human ,cattle, goat,, sheep and wild rodent are susceptible for infection.
IFA is probably the way to go for seroprevalence research. For in-depth analyses of exposure to Coxiella burnetii, also when antibodies may have reduced below detection level, the new interferon-gamma release assay for Coxiella may be helpful.
Although ELISA is widely used method for diagnosis due to its ease in performance but IFA is considered as a gold standard. So better to screen with both ELISA and IFA to get accurate diagnosis. If you require to select one then go with IFA.
IFA is the gold standard. However, ELISA may be convenient and easy. IFA requires more supplies and expertise compared to ELISA.
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