I am working in cell lines in which I have used 4 groups (Control, Toxin treated, Drug + Toxin, Drug alone). I need to find the significant difference between control vs toxin and toxin vs Drug + Toxin/Drug alone. Should I use independent t test or paired t test for such experiments.
If you want to compare only the means of two groups, student t-test is good, in your case, student unpaired/independent t-test. But if you want compare the means of more than two groups, you have to do with ANOVA. Please check the normality of data before proceeding to mean comparison.
For comparing in your work between different groups, We should go for ANOVA.
Ya its right. when you want to check more than two variables ANOVA is the better choice
Rather than the t-test you can go for ANOVA because of multiple variables in your case.
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