Hey, I'm Samavia Zafar. Currently, I'm working on multi-epitope subunit vaccine design and evaluation against Salmonella typhimurium. The paper which I followed for construction of multiepitope vaccine is "A candidate multi‑epitope vaccine against SARS‑CoV‑2 ".Link of this paper is attach here :[ A candidate multi-epitope vaccine against SARS-CoV-2 | Scientific Reports (nature.com) ].Adjuvant protein is attach at N-terminal of multiepitope vaccine construct.

1. My question is which sequence attach at N-terminal of CTB adjuvant protein?

2.How many amino acids attach at N-terminal of multiepitope vaccine construct?

3.Which strategy has been used for selection of amino acids?

4.CTB protein attach on which basis?

please send sequence of CTB which attach at N-terminal of amino acid.