Which solvent is best for etraction of phlorotannins from brown seaweed?

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What all temperatures should I set to perform Gradient PCR with different melting temperatures of forward and reverse primers?

Gene with FP having Tm= 63.5 and RP Tm= 64.5. So what all temperatures should I set for Gradient PCR??

02 March 2018 7,121 4 View

How to perform depression studies on cell lines and what are the genes responsible for depression like phenotype?

Cell lines used are SH S5 Y5 Is Dexamethasone Suppression test appropriate for inducing depression?

11 December 2017 1,966 2 View

How to perform silver nitrate test for arsenite (III) oxidizing bacteria? Or is there any other method for screening bacterial arsenite oxidization?

My bacterial isolates are capable to grow on 600 ppm concentration of arsenite (III) which is used as a electron acceptor by bacteria. For the confirmation of transforming ability of arsenite by...

11 December 2017 5,243 1 View

What is the optimum volume to be used in 96 well plate UV-Vis Photometer?

Path length and volume of the sample relationship in 96 well plate - Spectroscopy.

10 November 2017 9,524 0 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

Why methanol and sulphuric acid used in the analysis of polyhydroxyalkanoates (PHA) by GC-MS?

Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?

22 July 2024 1,210 2 View

When making abraxane with Nab tech, why is 9:1 Chloroform&EtOH used as solvents?

Is it the best optimization ratio based on experiments?

22 July 2024 8,170 0 View

What is the solvent system required to run tlc for calixarenes?

If you don't understand my question let me know

21 July 2024 563 1 View

Convergence of absorbency values to determine the total phenolic content of several solvents?

I used several solvents and measured the absorbency and determined the total phenolic content, but the results were similar after dropping them on the titration curve; What is the reason, please help

17 July 2024 5,151 5 View

How Can I Resolve a Persistent Unwanted NMR Peak at 1.25 ppm?

Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...

15 July 2024 9,065 4 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View