What is the optimum volume to be used in 96 well plate UV-Vis Photometer?

Gunaseelan Sathaiah @Gunaseelan_Sathaiah

11 November 2017 0 10K Report

Path length and volume of the sample relationship in 96 well plate - Spectroscopy.

Badges
Science topic

More Gunaseelan Sathaiah's questions See All

What all temperatures should I set to perform Gradient PCR with different melting temperatures of forward and reverse primers?

Gene with FP having Tm= 63.5 and RP Tm= 64.5. So what all temperatures should I set for Gradient PCR??

02 March 2018 7,060 4 View

How to perform depression studies on cell lines and what are the genes responsible for depression like phenotype?

Cell lines used are SH S5 Y5 Is Dexamethasone Suppression test appropriate for inducing depression?

11 December 2017 1,904 2 View

How to perform silver nitrate test for arsenite (III) oxidizing bacteria? Or is there any other method for screening bacterial arsenite oxidization?

My bacterial isolates are capable to grow on 600 ppm concentration of arsenite (III) which is used as a electron acceptor by bacteria. For the confirmation of transforming ability of arsenite by...

11 December 2017 5,215 1 View

Which solvent is best for etraction of phlorotannins from brown seaweed?

simple etraction method for phlorotannins.

01 January 1970 9,972 2 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View