Gunaseelan Sathaiah

5 Questions 6 Answers 0 Followers

Questions related from Gunaseelan Sathaiah

What all temperatures should I set to perform Gradient PCR with different melting temperatures of forward and reverse primers?

Gene with FP having Tm= 63.5 and RP Tm= 64.5. So what all temperatures should I set for Gradient PCR??

03 March 2018 7,107 4 View

How to perform depression studies on cell lines and what are the genes responsible for depression like phenotype?

Cell lines used are SH S5 Y5 Is Dexamethasone Suppression test appropriate for inducing depression?

12 December 2017 1,921 2 View

How to perform silver nitrate test for arsenite (III) oxidizing bacteria? Or is there any other method for screening bacterial arsenite oxidization?

My bacterial isolates are capable to grow on 600 ppm concentration of arsenite (III) which is used as a electron acceptor by bacteria. For the confirmation of transforming ability of arsenite by...

12 December 2017 5,232 1 View

What is the optimum volume to be used in 96 well plate UV-Vis Photometer?

Path length and volume of the sample relationship in 96 well plate - Spectroscopy.

11 November 2017 9,512 0 View

Which solvent is best for etraction of phlorotannins from brown seaweed?

simple etraction method for phlorotannins.

01 January 1970 9,995 2 View