Does using a likert scale in my qualitative research will make it mixed method?

I am conducting a qualitative research and using two qualitative data collection tools (1. Reflective journal 2. Semi-structured interviews). In the reflective journal I have asked the...

10 December 2020 4,672 7 View

In TEM sample preparation what quantity of nanoparticle needs to be mixed in ethanol before ultra-sonication? min quantity that gives results?

I have to send sample in another city for TEM analysis, they said send prepared sample..

25 June 2019 5,113 3 View

What is the minimum concentration of methanol extract of plant non-toxic to human cell-line? Should I use the extract and bacteria at the same time?

I am testing effect of plant extract on human cell line (hacat) infected with bacteria. My extract is prepared in methanol (10 g of plant in 100ml of methanol). What concentration of extract...

03 December 2018 2,169 3 View

Can anyone help me with the PLA blends thin film

I want to produce PLA blends by using solution casting method. I am using chloroform as a solvent. The steps are conducted in fume chamber. I have precondition the PLA in an oven at 70 deg around...

13 August 2017 5,987 2 View

Can restriction enzymes only be added to the end of a primer or is it possible to add it within the primer sequence?

Testing primers previously designed where restriction enzymes were located in the middle of the primer sequence but not sure whether this is actually possible.

02 May 2017 2,969 3 View

When doing antioxidant assays (plants), would one need to generate a standard curve for the specific assay?

I will be doing the DPPH. SOD, CAT , AP and GR assays and was wondering how I'd determine if there is any effect when comparing my plant samples subjected to different abiotic and biotic stresses.

17 March 2015 4,066 5 View

Does anyone works with tobacco pathogens? If yes, will you be able to provide me with it?

01 January 1970 3,675 4 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How long does it take to get a modeller(for homology modelling) license?

I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...

25 February 2021 9,054 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View