26 August 2016 3 3K Report

I need some practical sources to find out self-assessment and self-repair forms in foreign language teaching/learning?

More Sinem Atis's questions See All
Llyod WPM Medium that I prepared did not solidify what should I do?

I am trying to prepare Llyod WPM medium but it did not solidify and remained in gel form. I was only add 2.1 g of agar and 0.72 g of Llyod for 300 ml. What should I do?

28 September 2023 3,854 0 View

What is the reason for observing two bands of beta-actin after lipofectamine transfection of keratinocytes cells?

After the transfection, I blotted the membranes with commonly used beta-actin ab (sigma) in our lab. I saw 2 bands for the fırst time and I do not know the reason. Thank you for your concern. Sinem

25 December 2020 8,889 3 View

Histidine tag problem ?

Hello, I am trying to clone, express and purify a mammalian protein (enzyme) from E.coli BL21 using pet28 expression vector. The vector has N terminal His tag however, because of some cloning...

05 August 2019 7,251 10 View

Why Ni-NTA or Co-NTA columns don't bound to the His-tagged protein ?

Hello all, I have a C-terminal his-tagged protein around 70kDa that I want to purify using IMAC technique. I have used both Ni-NTA and Co-NTA columns from different companies and I have used both...

30 June 2019 6,992 16 View

How can I perform gravimetric analysis for glycine which is incorporated in my sample?

There are several samples which is believed that glycine is incorporated in them. I must know the exact quantitative amount of the glycine in the sample? What would be the most suitable way to...

17 June 2019 7,723 3 View

Plasmid lost after glycerol stock ?

Hello everyone, I am having a trouble in keeping my plasmid inside the bacteria. My plasmid is a Lentiviral plasmid of 7 kb and with the insert that I cloned is now 9 kb. The resistance gene is...

20 October 2018 9,373 4 View

Osmotic pump incubation before implementation at drying oven or cell culture incubator both sterile conditions?

Dear all, I am wonder if it is differ for activation of ALZET micro osmotic pump at drying oven ( like glassware sterilization ovens, 37 celcius degree) or typical cell culture incubator with...

23 September 2018 9,938 2 View

Have you experienced ALEXA 488 photobleaching?

Only 7 days later in dark and +4C storage, the signal reduced 60-70%, it seems unexpected. There may be two reasons related with cover solution including hoescht, or expire date of Alexa 488 dye.

29 July 2018 7,556 5 View

Glycine at pH 14?

I used glycine in a hydrogel network structure. From a bunch of hydrogels the ones that contain Glycine turn into pink/reddish color when they are kept in pH 14 solution for a couple of days. How...

03 April 2018 8,222 4 View

Urea and Lysozyme are compatible with each other or not ?

Hello, I am trying to purify a mammalian enzyme from bacteria E.coli (BL21(DE3)) and I have cloned the gene into pet28a and start to do induction with IPTG. After lysis with Lysozyme containing...

15 February 2018 701 6 View

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Need help identifying crystalline material precipitated from sour cabbage brine?

Hi.....I am working on a science project with my grandkids identifying crystals and have hit a real puzzler. We took some brine from a store-bought sour cabbage to see what might crystallize from...

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MorphoJ is not giving me the results of Canonical Variate Analysis. What I do?

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