What is the reason for observing two bands of beta-actin after lipofectamine transfection of keratinocytes cells?

25 December 2020 8,668 3 View

How can I perform gravimetric analysis for glycine which is incorporated in my sample?

There are several samples which is believed that glycine is incorporated in them. I must know the exact quantitative amount of the glycine in the sample? What would be the most suitable way to...

17 June 2019 7,560 3 View

Plasmid lost after glycerol stock ?

Hello everyone, I am having a trouble in keeping my plasmid inside the bacteria. My plasmid is a Lentiviral plasmid of 7 kb and with the insert that I cloned is now 9 kb. The resistance gene is...

20 October 2018 9,208 4 View

Osmotic pump incubation before implementation at drying oven or cell culture incubator both sterile conditions?

Dear all, I am wonder if it is differ for activation of ALZET micro osmotic pump at drying oven ( like glassware sterilization ovens, 37 celcius degree) or typical cell culture incubator with...

23 September 2018 9,799 2 View

Glycine at pH 14?

I used glycine in a hydrogel network structure. From a bunch of hydrogels the ones that contain Glycine turn into pink/reddish color when they are kept in pH 14 solution for a couple of days. How...

03 April 2018 7,997 4 View

What is the accepted limit of microbes and heavy metals in ready to consume fast foods?

What is the accepted limit of microbes and heavy metals in ready to consume fast foods?

09 October 2017 4,755 2 View

How to clean UV compatible 96-well plate ?

Hello, I am working with an enzyme kinetic activity in which the substrate is a fatty acid.. After I did my measurements I was cleaning the plate with deionized water but when I re-use it I am...

17 August 2017 8,066 5 View

Does expired human collagen(LYOPHILIZED) type IV work while coating plate?

The tube has net been opened yet.

02 April 2017 3,624 1 View

Is it possible to do sequencing after real time PCR or Is there any way to confirm the results of a TaqMan kit for real time PCR?

We are going to do real time PCR with TaqMan SNP Genotyping assay to determine the genotypes of a population. However we donot have a positive control. I am wondering that is it possible to do...

21 December 2016 8,775 6 View

How can I solve my western problem related to image quality?

After completing all steps including transfer, blotting and antibody staining, images obtained were as blurry. I added an image for an example. How can I increase the image quality? Is it related...

12 July 2016 8,716 11 View

How to gate mouse MDSC cells through flow cytometry?

On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...

27 January 2021 608 2 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

Are these IPScs Differentiated ?

Hi everyone, So these are images of IPSCs 2 days post passaging/splitting. I noticed that the overall morphology of colonies are not compact or round and without distinct border. However, at...

28 December 2020 3,503 3 View

How to perform the SEM, TEM characterizations to study the surface morphology of a Gold-coated PCF sensor?

I have designed an SPR based PCF sensor, which has a TiO2 and Gold coating. A reviewer has asked for the characterizations (e.g. SEM, TEM) to study the surface morphology. The reviewer has also...

06 December 2020 7,210 3 View

How we can isolate" distinguish" between Tyrophagus putrescentiae and T. entomophus ?

If I want to isolate Tyrophagus entomophagus by selective media, as I know they can grow on molds of stored grains,fruits, cheese. Or even in beehives debris. But how can I got only one species...

30 November 2020 9,087 3 View

How to calculate Lexical frequencies and Text Frequencies of phonemes and graphemes of English language?

This is so far the procedure I was trying upon and then I couldn't fix it As per my understanding here some definitions: - lexical frequencies, that is, the frequencies with which correspondences...

27 September 2020 4,742 3 View

Is there a website specialized in online speech data collection?

Collecting acoustic data during the pandemic faced me with a challenge that I need to overcome. I have to upload some audio files and scripts, and the subjects are required to answer the questions...

22 September 2020 4,168 6 View

Can you help me explain these morphological changes in Human fibroblasts?

Hello I have these human fibroblast cells in culture for a while and they look fine with a typical fibroblasts morphology, At passage 8 the culture looks like a heterogenous culture with...

22 September 2020 7,856 3 View

What can we call this type of surface morphology formed in Cu2O at high laser power during the SLM process?

A kind of surface roughness is produced by the application of laser energy density at high laser power (> 140W) which looks like an open porous structure. This kind of structure is not observed...

18 August 2020 9,835 4 View

How may untypical structures inside the spores of arbuscular mycorrhizal fungi be identified?

I can not describe morphology of some AMF spores isolated from soil sample. Permanent slides in PVLG+Melzer, x600, light microscopy and phase-contrast microscopy. Pink stained spherical structures...

01 August 2020 3,498 3 View