At the risk of some false positives, I want to maximize initial detection of a subgroup 1a rhabdovirus. Which has the higher sensitivity, semi-nested RT-PCR with degenerate primers or rRT-PCR?
Hello Annie,
though both are good choices, based on my experience, i always feel real time RT PCR has better sensitivity than conventional RT PCR , also has better specificity due to less handling .
on other hand Semi nested RT PCR has better sensitivity than just first round RT PCR. But then as handling increases during reaction set ups, chances of contamination also increases.
Hello Annie,
though both are good choices, based on my experience, i always feel real time RT PCR has better sensitivity than conventional RT PCR , also has better specificity due to less handling .
on other hand Semi nested RT PCR has better sensitivity than just first round RT PCR. But then as handling increases during reaction set ups, chances of contamination also increases.
Dear Colleagues,
Perhaps my thanks better late than never? I was remiss in checking you responses and am grateful for them. Your opinions fortunately validate my decision following the lit review.
Many thanks,
Annie
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