Annie Wagener

2 Questions 5 Answers 0 Followers

Questions related from Annie Wagener

Which RT-PCR-based assay will maximize detection for a virus?

At the risk of some false positives, I want to maximize initial detection of a subgroup 1a rhabdovirus. Which has the higher sensitivity, semi-nested RT-PCR with degenerate primers or rRT-PCR?

30 October 2014 108 3 View

Can either a seminested RT-PCR or rRT-PCR(EF1-α-specific assay) replace virus isolation as the screening test for viral fish pathogen surveillance?

Does either seminested RT-PCR (w/degenerate primers/Taqman probe) or rRT-PCR ( w/Taqman probe/AF 485331 EF1-α-Cyprinus carpio) have a reliably higher diagnostic/analytical sensitivity than virus...

27 October 2014 6,804 4 View