Hi Mohammed,

I used several kits/methods to extract RNA from "difficult" samples (e.g. mosquito legs). I found kits to be very similar in RNA yield and integrity, i liked the SV total RNA extraction system (Promega) and the Quick RNA (Zymo Tech) while i personally don't like the RNEasy family (Qiagen).

Anyway, the best RNA extraction methods are definitely the traditional phenol chloroform method or, even better, the Trizol-based method (https://medicine.yale.edu/keck/ycga/Images/TRIZOLRNAIsolation_092107_tcm240-21453.pdf). They require more time than using kits, but my RNA concentrations and purity were always far better than with any kit and i always had very good total (2x150) and smallRNA sequencings with Illumina HiSeq and NovaSeq.

Also check this article, it may be useful to decide an extraction method based on your sequencing target/application.

Article Comparison of RNA isolation methods on RNA-Seq: implications...

The best kit to extraction NA( DNA and RNA) is kit has made by USA company (Promega) . Good Luck.

RNeasy Plus Kits give you high yields of high-integrity RNA in minutes for any downstream application, without the need for DNase. No matter which cell or tissue type

https://www.qiagen.com/ca/products/discovery-and-translational-research/dna-rna-purification/rna-purification/cell-free-rna/rneasy-plus-mini-kit/#orderinginformation