Have you ever seen that 40S and 60S of ribosome subunit peaks disappear from polysome fractionation?

I am doing polysome fractionation for stress conditions for mammalian cell. it seems that peaks of 40S and 60S disappear during stress condition compared to control. I am wondering whether all...

12 November 2018 3,513 2 View

How is possible to find out time-displacement table from cyclic loading diagram?

Hi How is possible to find out time-displacement table from cyclic loading diagram ? or is there any way available to extract this information from hysteresis plot ? I mean , i wanna extract...

13 January 2018 9,220 4 View

Why might my neurofilament IHC not be working?

I am using the Abcam antibody ab4680 on mouse brain tissue fixed via PFA perfusion. The protocol we are using is the same as in http://dx.doi.org/10.1016/j.cell.2015.11.025. It's a staining method...

23 December 2017 7,063 3 View

In your experiance with the HER have you encountered a condition/material where the reaction order vs. H+ concentration is 0.5?

I'm wondering if anyone knows any experimental scenarios where the reaction order for the HER is 0.5 (vs. pH.), either acidic or alkaline environments. It is very common to see values like 1, 1.5,...

23 December 2017 766 1 View

What is the reason for change in mRNA level of specific gene but not change in protein level?

With treatment of cell lines ( cardiofibroblast cells), changes in mRNA level of the gene of interest has been observed overtly, but there is no change in protein level. I am wondering how this...

19 August 2017 1,026 6 View

What is the effect of immidazole on activity and stability of enzyme?

When enzymes were eluted with high concentration immidazole, some of them lost their activity in 2 days. I want to know how immidazole affect enzyme or other protein. I can't find article about that.

19 January 2015 7,283 6 View

How can I solve the low speed of my simulation in simulink?

I have a multi machine power system, and I had to simulate it by solving differential equations of the whole system in simulink, but the speed of the simulation is very low, how can I do this...

28 October 2014 7,921 15 View

How can I determine ph stability of my enzyme?

I provide mix buffer containing 100 mM glycine, 100 mM tris, 100mM succinic acid and 100 mM imidazole with ph 3, 3.5.....9 25 microlitr enzyme in tris buffer with ph 7.5 was mixed with 50...

13 September 2014 124 19 View

Why does the PH of tris-base buffer change with temperature variation?

I use Tris-HCL as lysis buffer for thermophilic enzyme. Is it possible the PH of this buffer changes when I use high temperature for enzyme assay?

06 August 2014 1,557 14 View

Why were recombinant proteins expressed in different OD of bacteria?

09 July 2014 9,197 12 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View