What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Can any one please suggest me that for micropore analysis of activated carbon which model gives relatively accurate value ?

I am new to micro-pore analysis. Recently, I have carried out low pressure isotherms (N2 @ 77 K and Ar @ 87 K) on activated carbon using a Quantachrome instrument. However, when I chose the...

21 April 2019 2,699 4 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

28 August 2018 4,866 2 View

Does anyone work with Diniobium Carbide (Nb2C)?

I would like to know if material is electrical conductive on nanosize domains. How different it is from NbC is Nb2C? Any literature?

19 June 2018 3,091 2 View

Is it possible to create a part from an orphan mesh?

14 June 2016 1,343 3 View

Attached is an image of PPF stained with PTA. The PPF has nano cellulose particles. My question is this artifact from the stain?

Stained with PTA for 15 minutes. Bulk material is PPF with nano cellulose crystals.

22 May 2016 1,559 1 View

No title

01 September 2014 7,367 4 View

Anybody to assist with HPLC software?

My laboratory received a donation of Waters HPLC system (Detector 2487, Multi-solvent delivery system, 600E series) from Seeding Labs, Boston last year but the equipment is not in use due to need...

30 October 2011 7,127 8 View

formaldehyde crosslinking for protein-protein interaction

Hi all, I'm trying to set up a protein-protein binding assay using formaldehyde (FA) crossslinking. I used formaldehyde before for RNA-protein binding experiments so I've pretty much reproduced...

21 March 2010 8,414 2 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

Extraction of phytochemicals from juice?

I am trying to extract phytochemicals from cane juice. I have tried lyophilization and SALLE techniques but the final product is too sticky and sugars are hindering any further extraction. I have...

03 February 2021 8,784 3 View

Does the UV radiation affect on fruits ?

I want to examine a kind of fruit's extract's antimicrobial effects. At first I want to put it under the UV radiation to prevent the mold or any kind of fungi's and bacteria's growth. The...

24 January 2021 8,350 20 View

What are the factors responsible for non uniform decrease in corrosion rate?

I am working on the corrosive effect of food condiments juices on some selected metals using weight loss experiment. But while plotting the graph, I found out that the decrease in corrosion rate...

21 January 2021 6,688 4 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Can any one suggest the name of Litchi insect/predator?

Is it a type of Ambush bug or what? Thanks

14 January 2021 9,381 3 View

Can I make a meta-analysis with different variables in the same forest plot?

14 January 2021 1,733 4 View