I have been looking for information on lipid quantification in TLC plates.
I would like to use malachite green but protocols pair it with perchloric acid. Using the latter is not an option at the moment.
Other protocols refer to phosphoric acid coupled with a different reagent.
As I cannot find info on the differences of action between these 2 acids upon the lipids in the charring process, would any of you know whether I can pair malachite green with phosphoric acid? Or would you have another new suggestion?
Thank you.
Why not use HPLC instead of TLC ? If the proper method(s) are used, it is far more selective and accurate.
Thank you for your answer. although LC-MS is available, we are now interested in pursuing HPTLC.
Depends on what is your target, have a look on this.
Afr. J. Food Sci Vol. 9(7), pp. 400-405, July, 2015
Thank you for your answer. would you know the minimal concentration or mass detected by the 10% phosphomolybdic acid that you used? Cyril Ogbuanu
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