How should the scholar working in the laboratory and in field based activities under a Project be handled by Principal Investigator?

All the externally aided projects which are funded have to go through rigorous checks and appraisals on a continued basis depending on the work plan. In order to meet the timeline the scholars...

31 May 2020 9,111 4 View

How can deforestation and anthropocene impact the biodiversity and lead to incidence of pest and diseases?

It has been alarming that human activities had increased many proportions and had led to many anthropogenic activities which had been directly and indirectly affecting the natural ecosystems....

29 April 2020 7,424 1 View

Why conventional breeding which has a much better role to play in genetic improvement of trees is not given much importance in research?

It is seen that conventional breeding is losing its charm in research as compared to modern biotechnological methods even though it is not preferred in terms of its field deployment. Moreover, if...

26 March 2020 3,225 20 View

What is the difference between shaking the PCR tubes with hand and rotor?

Can anyone give the scientific explanation.

12 March 2020 7,337 2 View

IGD indicator, GD indicator python or julia (MOEA)?

Hello all, Does anybody know any framework/code for IGD indicator, GD indicator in python or julia programming?.Also Is it ok to use a hypervolume indicator when it is biased with respect to...

30 July 2019 2,087 3 View

What is flexion point of a polymer?

How the flexion point is related to the mechanical strength properties and degradation of a polymer?

27 February 2018 5,081 1 View

What types of cathode lamps has to be used in AAS ie. coded or noncoded lamps?

i has to purchase AAS lamps in that what should i will buy coded or noncoded cathode lamps. what is difference between these two.

30 November 2017 2,856 4 View

Can anyone one help on the issue regarding determination of peroxide value?

04 January 2016 9,921 2 View

Formula for calculating crystallite size and lattice strain using XRD data?

What is the standard procedure to calculate crystallite size and lattice strain using XRD data. How accuracy the Scherrer formula is. Can I use it to calculate crystallite size and also How about...

14 November 2014 7,864 10 View

How can one identify the topological switch point in proteins?

13 November 2014 9,357 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View