Which protocol is more appropriate for site directed mutagenesis in studying fungicide resistance?

Anastasios A Malandrakis @Anastasios-Malandrakis

29 January 2015 1 9K Report

I am working with fungicide resistance mostly caused by target site modifications and in most cases SNPs are in fact responsible. I would be interested to insert such SNPs in sensitive fungal isolates to validate if they are trully resistance mutations. Since I have no former experience in this subject I would like to know which protocol/kit I should try. Fungal species I work with include B. cinerea, Alternaria Alternata, Penicillium expansum and Cercospora beticola. Any experience/ideas?

Thank you very much on advance.

Suseelendra Desai

One of the time tested methods is using short-wave UV source.

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