Which probes are better to check the expression pattern ? Oligonuclieotide Probes or RNA Probes?

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Almas Shamaila

Is it that oligonucleiotide probe of 100bp are more specific and reduce background staining

Manoj Kumar

RNA based probes are better because RNA-RNA hybrids are more stable.

Valentino Costabile

Moreover decrease the number of false-positive being more specific for designed target and not annealing different patern.

Subramaniam Sivakumar

Oligonucleotide probes are better than RNA probes

Rana M. Jamous

Oligonucleotide probes are generally less sensitive than RNA probes since fewer labeled nucleotides can be incorporated per molecule of probe