when i am growing the Dalbergia sissoo seed in vitro after sterilization into Half-strength of MS media then it grown well but some seeds leaves are brown under the test tubes.But when i shifted into callus media contain Kinitin and 2-4-D then after 3 days ,pink clr dots contamination formed around the ex plants but it did not spread whole petri plate just around the ex-plants. what will be the solution to remove or control the contamination
If the pink or brown colour is not spreading than there may be possibility of seed exudates coming out of seeds. However pink colour spot may be the bacterial infection that can spread. Application of bactericides in to media may control the
contamination.
You mean that you are doing seed culture not embryo culture? However if prior culture the seed surface is properly sterilized (e.g. using for example CaClO4 etc. ) then just add low concentration of general fungicide in the culture media and the problem might be solved.
There must be some other opened old test tubes in your lab or some other stuff like that. Secondly, must take in account the humidity where you are working, sometimes, that is more suitable for fungus rather than your ex-plant. Thirdly, don't ignore a minor mistake in sterilizing ex-plants.
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