Which Polymerase should I use for obtaining a gene through PCR which is 3Kb long?

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No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

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Bashiru Garba

For a 3kb DNA fragment, the usual Taq DNA polymerase will be sufficient (we have used it successfully). However, other proofreading polymerases for much longer sizes can be used including GoTaq® Hot Start Polymerase by Promega. Pfu polymerases are rather high-fidelity polymerases where extremely pure DNA is required for downstream application like cloning and mutagenesis

Debmalya Roy

Better to use KOD one (TOYOBO) or KOD plus (TOYOBO).

The amplification rate is 1kb/10 sec for KOD plus, very fast, and has strong 3'→5' exonuclease (proof-reading) activity with high fidelity.

Junjie Shao

Neb Q5.