Hi all
I am trying to sequence a 10kb DNA amplicon. The amplicon was generated from cDNA with long-range PCR.
I want to sequence the 10kb DNA using Oxford nanopore but I am not sure which protocol should I use and which sequencing kit is best for me!
I have the Ligation Sequencing Kit SQK-LSK110. There are many different protocols in the ONT website related to this sequencing kit such as:
- Genomic DNA by Ligation
- Lambda Control Experiment
- Amplicons by Ligation
- Low input genomic DNA with PCR
- PCR barcoding genomic DNA
- PCR barcoding amplicons
- PCR barcoding (96) genomic DNA
- PCR barcoding (96) amplicons
- Premium whole genome amplification
- Exome sequencing
- Sequence capture
Which one should I use? is it the "Amplicons by Ligation" protocol?
According to the nanoporetech website (https://store.nanoporetech.com/ligation-sequencing-kit110.html) this sequencing kit should be used for singleplex samples. Does this mean that I can not sequence multiple samples at the same time using this kit?
Your help is highly appreciated.
If you are trying to pool multiple samples, you need to barcode them. And look for corresponding protocol.
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