To separate out rutin, extracted with ionic liquid (1-ethyl-3-methylimidazolium-bromide).
Dear Bimal Chitrakar please see the following article entitled "Optimization of Ionic Liquid Based Simultaneous Ultrasonic- and Microwave-Assisted Extraction of Rutin and Quercetin from Leaves of Velvetleaf (Abutilon theophrasti) by Response Surface Methodology". The paper is available as public full text on RG:
Article Optimization of Ionic Liquid Based Simultaneous Ultrasonic- ...
On page 9 of this article it is stated that "it is worth noting that [C4mim]Br and methanol were almost the same as the extraction yields of two target compounds. However, methanol was volatile, flammable, and harmful to human and environment. Therefore, [C4mim]Br was chosen as extraction solvent in this study." Thus I would try re-extraction of your rutin with ethanol or perhaps use methanol right away instead of the ionic liquid.
Dear Bimal Chitrakar please see the following article entitled "Optimization of Ionic Liquid Based Simultaneous Ultrasonic- and Microwave-Assisted Extraction of Rutin and Quercetin from Leaves of Velvetleaf (Abutilon theophrasti) by Response Surface Methodology". The paper is available as public full text on RG:
Article Optimization of Ionic Liquid Based Simultaneous Ultrasonic- ...
On page 9 of this article it is stated that "it is worth noting that [C4mim]Br and methanol were almost the same as the extraction yields of two target compounds. However, methanol was volatile, flammable, and harmful to human and environment. Therefore, [C4mim]Br was chosen as extraction solvent in this study." Thus I would try re-extraction of your rutin with ethanol or perhaps use methanol right away instead of the ionic liquid.
Bimal Chitrakar P.S. I don't know how methanol and your ionic liquid are miscible. I suggest to just make a few quick extraction experiments instead of waiting for more answers. Why did you use the ionic liquid anyway when methanol works equally well? What are the advantages of the ionic liquid?
One possible solution could be the following: Rutin is known to be soluble in aqueous alkali. Thus you could try to re-extract it with aqueous NaOH solution (although I don't know how this is compatible with the ionic liquid). Just give it a try. If the rutin dissolves well in aqueous NaOH, you can perhaps isolate it by acidifying the solution.
P.P.S. This would be a good question for our flavonoid expert Yasser Fakri Mustafa 😊
Dear Bimal Chitrakar, rutin is highly polar molecule therefore it can be extracted by using ethanol and IPA, but i suggest onetime check extraction in Diethyl ether.
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