Sreshtha Das

6 Questions 6 Answers 0 Followers

Questions related from Sreshtha Das

Which of the following is autoclavable and required autoclave- EDTA, CTAB buffer-Tris-Hcl,, sodium acetate buffer , ctab extraction buffer ?

it wil be helpful to get ctab extraction buffer components needclike how much pvp .

15 June 2025 7,507 4 View

How to decide on the dilution factor for qPcR?

In one text (wallace and Rieske 2023) I found this "Quantitative PCR was then performed on a 5-fold dilution of cDNA (starting at a 1:25 dilution) "while on the other paper (Sellamuthu et al.,...

26 March 2025 5,134 2 View

How not to get a smeared and low intensity band even after using the kit for bacterial genomic dna extraction? what is the problem in lane 3?

I am using Qiagen Dneasy blood and tissue kit for bacterial dna extraction , what mistakes i am making is it just pipetting or a problem in gel prepararing and putting into the buffer tank to...

13 March 2025 5,169 3 View

How the ratio of DNA sample : dye of 6x loading dye has effect on the bands we observe via Gel-Doc?How dilution matters?

I have been running agarose gel electrophoresis and visualizating the gel after the electrophoresis.I did dilution like I took sample 10 microlitre and 2ul 6X loading dye ;total loaded 12ul in...

05 March 2025 8,500 0 View

How to do RNase A decontamination ?

I have been using the QiagenKit and using the RNas A as mentioned in the protocol but still getting RNA contamination #DNA extraction, Decontamination, Qiagen

27 February 2025 6,679 2 View

How to remove RNA contamination?

I am following protocol of Qiagen Blood and DNAeasy kit and still getting RNA contamination.Should I add RNAseA after the elution?

25 February 2025 3,721 7 View