Yes if you sterilized it by microfilter after autoclave sterilization.
Autoclaving may partially degrade growth hormones in the medium, however, media recipes usually account for that loss. So it depends how precise you want to be with knowing the accurate level of the phythormone available to plants from the medium.
Dear Rahmand
I agree Arman & Anna, although TDZ is unstable at high temperature, it add autoclavable and must be sterilized by ultrafilteration inside hood at room temperature, especially in case of add it in a certain concentration
good luck with best regards
I agree with the others who have suggested filter sterilization. I think this is a much safer method to minimise damage to the hormone. Autoclave the media, then add the filter sterilized hormone in a sterile flow hood.
As a general rule, growth regulators should be added after autoclaving the basal medium under sterile conditions. The stock of the growth regulators must be kept ready by ultrafilter sterilization. This is important because growth regulators are used in micromolar concentrations and experimenters cannot afford to allow growth regulators to partially degrade. Therefore, I recommended it as a general rule.
Thidiazuron is sensitive to heat, so that you must add it after autoclaving stage. You can solve it in DMSO, then add to medium culture.
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