Which normal neuronal cell line will be best to study its activity with/on Dipeptide scaffold?

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MiRNA:mRNA alignment???

Based on literature review and existing sequencing data, we chosen few miRNA, then we over-expressed them in cancer cells using miRNA mimics. We found a gene expression getting down-regulated in...

07 August 2018 4,236 0 View

Ethical way of citation?

Now a days it become hot topic of discussion that how to give relevant, accurate and exact citation to any particular paper or article for some example like MTT, protein estimation by Lowry ,...

07 August 2016 7,151 2 View

How long we can use any T25 flask for cell culturing?

For example my cells are growing slow, gaining confluency along with attaining morphological growth is taking time in a T25 flask. I am keep on changing media after every 48 hours. So my question...

05 June 2016 6,703 5 View

Can anyone provide me SAXS measurement protocol for dipeptide hydrogel sample?

I have synthesized peptide having two amino acid derivatives. I am preparing hydrogel from that peptide after putting them in particular solvent system. And, I want to do SAXS measurement for that...

31 December 2015 2,923 3 View

Could anyone suggest step by step procedure to process the SEM image using ImageJ software to study pore size of the nanofiber hydrogels?

I have SEM image of my nanofiber hydrogel. they are like a network forming ellipse type pore. I want to determine say calculate pore size? i want step by step procedure to use imageJ software so...

10 November 2015 4,449 2 View

Can anyone suggest some journals which publish articles related to synthesis and characterization of silver nanoparticles using plant extract?

Impact factor of journal should be at-least (0.1-0.5). please help me out?thank you

04 May 2015 4,751 4 View

Any suggestions on an absorption kinetics study of Carbamazepine drug removal from water by magnetic nanoparticle capped with plant extract?

I am performing absorption study: carbamazepine drug removal using plant extract capped magnetic nanoparticle (MNPs). In that 5, 10, 15, 20 ppm of drug concentration and 50mg of MNPs I am using....

03 April 2015 6,842 0 View

Will sonication of our plant extract capped magnetic nanoparticles affect batch absorption for removal of selected pharmaceuticals from water?

Is it necessary to sonicate our plant extract capped magnetic nanoparticle sample for batch absorption study for the removal of selected pharmaceuticals from aqueous solution or we can used...

02 March 2015 1,805 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View