MiRNA:mRNA alignment???

Based on literature review and existing sequencing data, we chosen few miRNA, then we over-expressed them in cancer cells using miRNA mimics. We found a gene expression getting down-regulated in...

07 August 2018 3,902 0 View

Ethical way of citation?

Now a days it become hot topic of discussion that how to give relevant, accurate and exact citation to any particular paper or article for some example like MTT, protein estimation by Lowry ,...

07 August 2016 6,993 2 View

How long we can use any T25 flask for cell culturing?

05 June 2016 6,554 5 View

Can anyone provide me SAXS measurement protocol for dipeptide hydrogel sample?

31 December 2015 2,773 3 View

Could anyone suggest step by step procedure to process the SEM image using ImageJ software to study pore size of the nanofiber hydrogels?

I have SEM image of my nanofiber hydrogel. they are like a network forming ellipse type pore. I want to determine say calculate pore size? i want step by step procedure to use imageJ software so...

10 November 2015 4,291 2 View

Can anyone suggest some journals which publish articles related to synthesis and characterization of silver nanoparticles using plant extract?

Impact factor of journal should be at-least (0.1-0.5). please help me out?thank you

04 May 2015 4,605 4 View

Any suggestions on an absorption kinetics study of Carbamazepine drug removal from water by magnetic nanoparticle capped with plant extract?

I am performing absorption study: carbamazepine drug removal using plant extract capped magnetic nanoparticle (MNPs). In that 5, 10, 15, 20 ppm of drug concentration and 50mg of MNPs I am using....

03 April 2015 6,710 0 View

Will sonication of our plant extract capped magnetic nanoparticles affect batch absorption for removal of selected pharmaceuticals from water?

Is it necessary to sonicate our plant extract capped magnetic nanoparticle sample for batch absorption study for the removal of selected pharmaceuticals from aqueous solution or we can used...

02 March 2015 1,666 1 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View