Hi there,
I am wondering, do any human/mouse skeletal muscle cell line resemble in vivo muscle structure better than others? If so, are there any limitations or things to think about while growing these cells in vitro?
Many thanks,
- Jelle Bonthuis
Dear Jelle Bonthuis,
Skeletal muscle cell line it is possible to use and can be cultured using the media mentioned above:
DMEM has almost twice the concentration of amino acids and four times the amount of vitamins that EMEM, as well as ferric nitrate, sodium pyruvate and some supplemental amino acids. The original formulation contained 1,000 mg / L of glucose and was originally reported to grow embryonic mouse cells. An extra variation of 4500 mg / L glucose has been proven optimal for the cultivation of a variety of cells. DMEM is a basal medium and does not contain any proteins or growth promoting agents. It is commonly supplemented with 5 to 10% fetal bovine serum. The DMEM uses a sodium bicarbonate buffer system (3.7 g / L) and therefore requires artificial levels of CO2 to maintain the required pH. The powdered media is formulated without sodium bicarbonate because it tends to be removed by gasification in the gaseous state. Powder media requires the addition of 3.7 g / L of sodium bicarbonate at the time of dissolution in water. DMEM was initially used for the culture of mouse embryo stem cells. It has been found to be widely applicable in studies of mouse and chicken primary cells, viral plaque formation and contact inhibition.
Best regards
Thank you!
Although I was wondering more about the different cell lines themselves, this will be usefull as well.
Have a great day
The C2C12 skeletal muscle line is a widely used mouse myotube culture. They're robust and easy to work with. Sorry, I can't speak to the in vivo structure. My understanding is that anything grown in a dish will be limited in how it resembles the same tissue in vivo.
Good luck!
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