Jelle Bonthuis

3 Questions 6 Answers 0 Followers

Questions related from Jelle Bonthuis

How does Crispr/Cas9 donor (template) DNA reach the nucleus of cells?

When repair guiding DNA is transfected into cells, how does it reach the nucleus to aid in the repair of DNA breaks? On a side note, say I transfect cells with Cas9 mRNA and template DNA, would...

12 December 2017 3,222 5 View

Experiences with inducing in vitro disease-models?

Hi there,I would like to induce some genetic diseases (Freidrichs, Duchenne etc.) in vitro, this is however my first time trying such a thing. Are there reasons to choose shRNA, siRNA over Cas9...

12 December 2016 9,964 2 View

Which mouse/human skeletal muscle cell line is best used in vitro?

Hi there, I am wondering, do any human/mouse skeletal muscle cell line resemble in vivo muscle structure better than others? If so, are there any limitations or things to think about while growing...

12 December 2016 4,880 3 View