Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

How structure of monomers in molecular imprinted polymers influence their selectivity?

Hello, I'm biologist and currently working on MIP. I wanted to know is there any literature that mention the influence of monomer structure (for example size of molecule) on MIP selectivity and...

27 October 2020 1,571 6 View

What mask can be used for ultra-deep KOH etching on 110 Si?

Hello everyone! I need to etch Si to the depth of 1mm approximately, not affecting the surface of the sample (which is atomically flat and must be kept as it is, so we can't anneal the sample)....

16 August 2020 9,727 1 View

Are there any papers where advantages and disadvantages of different drug delivery systems are observed?

I'm writing PhD thesis and need to compare different drug delivery systems applied to SOD delivery.

22 July 2020 7,210 2 View

How to calculate the band structure for a two-dimensional triangular lattice?

You can provide links with calculation and clear examples of the methods used. I would also like to understand how to take into account a specific potential. How to determine the forbidden zone...

09 June 2020 9,728 2 View

Can anyone advice empirical papers on ideas and their impact on institutional change?

Does anyone know any good papers on the impact of the political elite's ideas on institutional transformations in terms of comparative politics? It will be really great if there are both...

27 February 2020 3,665 3 View

Could someone recommend a journal similar to Biodiversity Data Journal?

I'd like to publish some ideas about taxonomical database. This is possible to do in Biodiversity Data Journal or PeerJ, but I need to pay for an article. Does anybody know similar journals...

23 February 2020 6,876 3 View

Is "green energy" really "green"?

Is "green energy" really "green"? Is Green Energy Really Eco-Friendly? Isn't solar panel manufacturing too dirty? Is it not too damaging to put wind turbines in farmland? Don't we pay too much...

24 January 2020 4,067 14 View

Is it possible to purify the protein using Ammonium Sulfate precipitation only?

Dear All, I am about to start purification of many variants of not tagged recombinant protein expressed in E.coli. I need it to be at least 70% pure. Column purification would be very time...

09 January 2020 7,116 4 View

What solutions (fixatives and dyes) are used to make colored sections of plant roots in mycorrhizal symbiosis destined for light microscopy ?

I want to make sections of mangrove roots to search for mycorrhizal symbionts. Which solutions are usually used for the fixation and dyeing of this kind of samples ? The fixative agents may not...

20 December 2019 7,362 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Is there an appropriate CD8 antibody for IHC-P for staining human breast cancer tissues?

Hello, We are planning on staining Formalin-fixed paraffin breast cancer tissue sections (FFPE) with CD8 Ab via immunofluorescence (Tissue IF). Based on our existing protocol and imaging...

24 February 2021 9,578 4 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View