Which methodologies are the most appropriate for the determination of the activity of the enzymes related to the Nitrogen metabolism?

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Ahmad Ali

GS can be assayed spectrophotometrically. Rest other enzymes can be measured by HPLC like techniques.

You can refer the following paper for protocols for nitrate assimilating enzymes

Ali, A., Jha, P., Singh, K. S., Raghuram, N. 2008. Spirulina nitrate-assimilating

enzymes (NR, NiR, GS) have higher specific activities and are more stable than those of rice. Physiol. Mol. Biol. Plants. 14(3): 179-182.

Carlos E. Aucique-Pérez

Dear Ahmad, thanks for your information.