Dear researchers and scientists,
I would like to clean some of my diatom samples, while i am checking for the procedure i came up with lot of procedure which make me confused.in some methods it requires foom hood which i dont have easy access here. can i get one best method which which you might have tried gives best result.
Thanks in advance.
Elaya:
Have a look at this useful method:
https://www.mccrone.com/mm/the-collecting-cleaning-and-mounting-of-diatoms/
Best
Syed
If you want to clean a sample collected from nature just to have a look at the various diatoms it will be best to try out the different methodologies given in text books. Most of those methods will have prescribed rigorous cleaning in the process even damaging the weaker ones with thin siliceous skeletons.
I would suggest that you modify the methods to suit your requirement. Always go for lesser strength acid. Ensure that you take samples at various points of the cleaning process. Chances of getting clean and unbroken samples will be greatly enhanced if you follow this advice.
My own experience that gave me good results is to have the diatoms in 6N HCL (approximately 50% strength acid). The samples were left in a test tube with a marble on top. Every other day you may shake the sample mildly and allow it to stand as earlier. Within a week you will notice that the precipitate at the bottom would have turned white. Wash the sample repeatedly in distilled water and prepare a slide as given in any standard methods and observe under the microscope.
Dear Syed sir and Valsaraj Sir,
Thanking you so much for your suggestion..
it is very much useful for me..
With Thanks and Regards...
U.Elaya Perumal.
You may try a filter an UV lamp.
Cleaning the culture bath every day.
G
I would suggest that you cleaned the diatoms with H2SO4 HNO3 at equally volume
Have you tried to culture them in agar? You prepare the agar plates with f/2 media and pour 1 mL of your diatom cultures, all under sterile conditions (a Fisher burner will be enough, but avoid windy places). Not all the species like this, but it is worth to try. After you pour the mL, you spread it with a bacteriological handle and in about 10 days you will be able to see the diatom colonies. Then you take only one colony and seed it in a tube with growth media. This is the way I used to mantain my diatoms cultures for many years. Hope this is useful.
Check it out
http://nzetc.victoria.ac.nz/tm/scholarly/tei-Bio26Tuat01-t1-body-d5.html
All best
Valsaraj cp a what will be my sample size for that, and how much acid i need to add. If i am using 0.1 ml for study, how much acid i should add for cleaning.
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