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Quality assessment of entomophagous arthropods used in augmentative biological control is one of the main concerns after their mass production. Economic production and quality are two important parameters that affecting biological contorol. Results from experiments with different kinds of diets with or without insect components, developed for parasitoids and predators, are examined. Morphological traits as well as development and reproduction parameters used for comparisons between in vivo- and in vitro-grown arthropods are discussed. Morphological characters include body size and weight and the occurrence of abnormalities. Immature development is assessed by measuring duration and survival rates of the different stages. Sex ratio and symbiont association, fecundity-fertility and longevity are compared as reproduction parameters. It is important to consider biochemical parameters, such as protein, lipid and carbohydrate content, for quality control. These parameters may also indicate the deficiency or excess in a particular nutritional component. Behavioural and genetic parameters are considered as well. The establishment of relationships between certain parameters, e.g. between body size and fecundity or longevity, may help in simplifying quality control procedures. The ultimate quality criterion for an artificially reared natural enemy is its capacity to reduce pest populations, which can be evaluated by measuring the predation efficiency or the parasitization rate under laboratory or field conditions.