Which method is appropriate in order to perform DNA extraction of Toxocara eggs?

More Adel Spotin's questions See All

Provided that the shale conductivity termed here x increases with temperature, how could we compute it at reservoir temperature ?

one could use Waxman and Thomas (1974) work?

22 July 2024 6,637 0 View

Is there a difference between the curriculum and the style of the study?

A question about scientific methodology

13 July 2024 2,160 8 View

How Bpai (Branson dental operator posture assessment instrument is scored? How the total score is 194?

Hello Everyone, May I ask you to explain how the total score of BPAI is summed to 194? I know the scoring of modified BPAI well, but I need the original form. This is a link to the...

20 June 2024 7,284 0 View

How can biocatalysis be integrated into larger carbon conversion processes, such as biorefineries or waste valorization schemes?

biocatalysis can be integrated into larger carbon conversion processes, such as biorefineries or waste valorization schemes. The main question: HOW

18 May 2024 2,869 0 View

What are the environmental benefits of using biocatalysis for carbon conversion compared to traditional chemical methods?

The environmental benefits of using biocatalysis for carbon conversion compared to traditional chemical methods are large. What are they?

18 May 2024 3,620 0 View

Are there any recent developments in the discovery or engineering of novel biocatalysts for carbon conversion?

Developments in the discovery or engineering of novel biocatalysts for carbon conversion cannot be stopped. we need to know any recent deevelopment in this field.

18 May 2024 5,345 1 View

What types of carbon sources can be effectively converted using biocatalysis?

Types of carbon sources can be effectively converted using biocatalysis

12 May 2024 2,389 1 View

What are the strategies for optimizing biocatalysts for carbon conversion?

For carbon conversion , the use of biocatalysts must be optimized, so what are the strategies used for this purpose?

10 May 2024 7,839 1 View

How efficient are biocatalysts in converting carbon sources into valuable products compared to traditional chemical catalysts?

The biocatalysts can be more available than traditional catalysts? The quation is related to the sustainablity by conversion of carbon using biocatalysts.

04 May 2024 9,761 2 View

If possible, practical exercises on Coherens numerical modeling program?

I need training videos about the program to learn how to use it in order to complete some research and student projects.

15 February 2024 4,197 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Sandip Chakraborty

You can please refer to the links provided below for obtaining certain valuable informations:-

1. http://www.parasitesandvectors.com/content/5/1/288

2. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3670902/

Adel Spotin

So thanks dear Sandip Chakraborty

Corrado Minetti

Hello!

by reviewing loop-mediated isothermal amplification methods for parasite detection I found the following paper you may find of interest. They used a very quick and simple DNA extraction method that you may want to try. Regards!

http://www.ncbi.nlm.nih.gov/pubmed/21158640

Thank your Dear Minetti

Steven Kenyon Ault

Contact Dr. Maria Elena Bottazzi, National School of Tropical Medicine at Baylor College of Medicine, Houston, Texas