Which method gives better result for crude lipid/fat determination, acid hhydrolysis or soxhlet extraction?

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Is there a universal weight for soil quality index under expert opinion?

There are several ways of estimating soil quality index, however the two most widely utilized are the 1) PCA method where the weights of the factor loadings are considered for the soil. 2) Expert...

02 April 2024 8,607 2 View

Unable to download land-sat 8 images from USGS website, any information?

I have been trying to download some Landsat 8 images for the past year. however whenever I add the mad to my cart and start the download, I always get this error The server encountered an...

18 May 2023 7,016 1 View

Structural equation model: does sem for soil require cfa?

I have read some literature where in the process of making sem for soil ecological purposes do not employ the traditional confirmatory factor analysis. May I kindly have some information on this?

08 May 2023 7,012 2 View

I am very interested in applying GIS/Remote sensing for soil ecological study. May I kindly have links to resources onto how to perform such a study?

I would love to hear from the researchers with experiences in incorporating soil and gis/remote sensing to monitor soil quality data.

07 November 2022 9,931 3 View

Steps to make biochar at home?

I am very keen in making biochar at home at small scale. A Cost effective method. I am very keen to know it's effect on garden produce. Any help or literature or methods would be greatly...

20 July 2022 2,517 3 View

Is there a more simpler method to determine organic matter content of soil that doesn't require a lot of equipments?

If there is, I would be extremely grateful to have some references. Thank you.

24 June 2022 7,094 3 View

Is it possible to incorporate fungal data to formulate soil quality index?

I have data's on SQI obtained from soil parameters alone. I wanted to know, if it was possible to incorporate fungal data (I have the total cfu of each sites) into soil quality index calcitonin...

19 April 2022 8,483 7 View

I am interested in building a SEM model for soila quality index, may I kindly have some advice/papers on this topic?

Structural equation modelling is key to understanding the internal workings of an ecosystem. I wanted to know if it's possible to build a SEM utilising data such as 1. Soil physical and chemical...

26 March 2022 5,922 2 View

What statsitical technique would best explain relationship between soil quality and yeild?

I have data's on both soil data (i.e. soil parameters like moisture, clay, texture, nok etc) and yield and agronomic traits of banana. 1) I wanted to know which statsitical technique would best...

24 February 2022 6,786 11 View

What might be the reason for low soil pH ( more acidic) during winter as compared to higher values(less acidic) during summer and autumn?

My soil data shows consistent lower pH values during the colder seasons (winter) eg 4.7 as compared to the warmer seasons with rainfall (summer autumn) eg 5.4. I have read alot of literature...

19 February 2022 4,778 9 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View