Moreover, do you first use a solvent (such as hexane or xylene) to remove the adhesive on the specimens, or do you work directly by taking the specimens from the sticky plate?
Thank you
We do not undertake any DNA extraction
I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....
31 July 2024 3,756 0 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Hi fellow researchers, I have got a noob query regarding the question of the post. I am trying to build a data set of enzymes (from UniProt data base) that would contain protein sequences and...
21 April 2024 7,281 4 View
As we know, lymph nodes have a mixed population of cells, how do we ensure only B cells are getting isolated and subsequently fused with myeloma cells? Thanks in advance!
30 January 2024 3,449 2 View
Hi, I need help processing for big data. I am trying figure out ''How does Twitter process big data? Also, what can be done with big data?'' Can you explain briefly and/or can you suggest...
05 November 2023 9,122 3 View
Why do ExpiCHO cells need 8% CO2 levels in the incubator? Is is because they have high metabolic rate, thereby producing more lactate and needing more CO2 to buffer the media? Would like to know...
26 August 2023 6,707 3 View
I am facing a problem while running brig with genbank files. I want to give gene annotations & following the manual I have added the genes with the start & stop codon mentioned. But after...
05 August 2023 5,601 0 View
I am facing a problem while running BRIG with genbank files. I have given plasmid sequences as my input files. I want to give gene annotations & following the manual I have added the genes...
05 August 2023 809 0 View
I am facing a problem to circularize a particular bacterial plasmid sequence while doing hybrid assembly in unicycler. After the assembly, it's not showing circular. While the other plasmids have...
29 July 2023 1,251 1 View
Also would like to know the ExpiCHO parameters like ellipticity/ circularity and max/min. diameter to count the cells via automated cell counters. Thanks for the help!
26 June 2023 904 0 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
...
24 July 2024 1,559 1 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
Is it the best optimization ratio based on experiments?
22 July 2024 8,170 0 View
If you don't understand my question let me know
21 July 2024 563 1 View
I used several solvents and measured the absorbency and determined the total phenolic content, but the results were similar after dropping them on the titration curve; What is the reason, please help
17 July 2024 5,151 5 View
Who can tell me in which year and in which journal the following taxa, nowadays placed in the family Erebidae, have been published: Simplicula Hampson, #### (Lepidoptera: Noctuidae) monospecific...
15 July 2024 9,192 10 View
Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...
15 July 2024 9,065 4 View