I am looking for an efficient tool for gene knockout in a gram positive bacterium..
Article Mini-Tn7 insertion in bacteria with single attTn7 sites
How can rural business building help to improve rural economy?
15 June 2024 3,462 3 View
I need lift coefficients of NACA 0030 for simulate DLD microfluidic behavior in cell separation.
10 March 2024 9,796 0 View
Hi. I need to remove all microplastics of a wastewater sample without changing any physicochemical properties of the sample. Can filtration by Whatman filters (for example Whatman 589/3 with...
08 March 2024 8,722 3 View
They are both used to differentiate gram negative organisms from gram positives. So what is the difference? Which one would be more beneficial?
19 February 2024 5,094 2 View
How Pseudomonas sp., Pectobacterium carotovorum and Ralstonia solanacearum strains isolated from the same host could be differentiated based on non-molecular methods (biochemical tests, culture...
12 February 2024 4,718 4 View
Is it possible to measure the amount of thermogenesis (turning white fat into brown) using its markers LIKE UCP-1 in the blood(PLASMA)?
29 November 2023 7,676 1 View
Presently, bio-absorbents have been widely studied by researchers. We plan to select a strong adsorbent based on waste materials to absorb urea and water. Therefore, I would appreciate the...
19 November 2023 3,857 5 View
I am a student of educational psychology
18 November 2023 2,583 5 View
What are the best primers for 16S rRNA sequencing for identification of plant-pathogenic bacteria in pure cultures? Which primers are the most common?
06 November 2023 5,351 1 View
How can I simulate laminar flow and particle tracing for DLD microfluidics in Comsol multiphysics?
16 October 2023 4,299 1 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?
29 July 2024 10,023 2 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View