I am looking for an efficient tool for gene knockout in a gram positive bacterium..
Article Mini-Tn7 insertion in bacteria with single attTn7 sites
Hello dear researchers. I want to solve a cylindrical waveguide, lick an ordinary standard optical fiber. I am familiar enough with the 2D-axis-symmetric model, but as I knew from the equations...
11 January 2021 6,663 6 View
I would like to make a beam splitter by a glass for a specific angle. First I calculated the thickness of the partially reflecting layer by using of the transfer matrix then I coated this layer by...
25 September 2020 7,283 1 View
What is the reason for the ineffective or insignificant effect of Neuromuscular relaxants such as Atracurium and anesthetics such as Ketamine on patients with Coronavirus hospitalized in the...
22 September 2020 5,734 4 View
Hi Im seeking advice on what would be the best model of T2D and metabolic syndrome in rats Thank you
04 September 2020 3,661 4 View
In visible reflectance spectroscopy performed on a bovine tendon, peaks were observed corresponding to tendon amides. But I do not know these peaks are related to what kind of amides.
16 August 2020 4,411 3 View
30 April 2020 906 2 View
Dear colleagues, Is it alright to register a systematic review and meta-analysis (focusing on human health studies) with the International Platform of Registered Systematic Review and...
25 March 2020 5,382 9 View
There are some crystals formed on the surface of the glass-ceramic substrate (mainly composed of Al, Si, Ca, O) after being treated with PBS (pH> ~10). I know that buffer salt crystalization is...
16 March 2020 4,385 3 View
Hi, Everyone. I am a PhD student and I need some data about fake news from twitter or any other platform that has available data. Can you please let me know how I can find data?
26 January 2020 6,415 12 View
I am going to study migration in a549 cell line after a treatment but the a549 cells which I am working with show no migration into the space created by a yellow tip even in normal condition (with...
13 August 2019 1,089 5 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?
28 February 2021 8,095 1 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View