How should I deal with missing data?

12 February 2021 5,306 4 View

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01 January 2021 9,970 2 View

How can I normalise cell count/protein concentration using SDS Coommasie blue stained gel?

Im quite new to cell culture and have had some inaccuracies while harvesting my cells and therefore counting them using a CASY counter. I reconstituted in what I thought to be 50,000 cells/ul,...

16 November 2020 9,829 1 View

Hazard value for malaria survival graph??

In an experiment untreated infected mice and infected treated mice observed. I recorded my data in terms of survived mice to 28 days in each group. How to calculate hazard value for treatment vs...

06 November 2020 5,548 1 View

What is the best blocking agent to block rGO biosensors?

I am trying to make a DNA sensor but I am detecting non specific binding. Does anyone know a better blocking agent for DNA sensors based on rGO, apart from BSA or MCH?

05 November 2020 5,140 3 View

No proteins of interest after Co-IP with Dynabeads?

Hello, I am trying to Co-IP my protein complex of interest, and every time i run it on the gel I get nothing but antibody bands. I conjugate my antibody to Dynabeads protein G overnight. I then...

29 October 2020 3,229 7 View

What kind of MMP should I use If I want to degrade rat tail collagen (Type I) gels?

I want to do MMP digestion on my collagen gels to see the effect of glycation on my collagen gels. I have difficulty to find the best MMP! some articles used MMP-1 some MMP-9 some MMP -2 and some...

28 July 2020 6,711 1 View

How can i test the Security for an algorithm in the field of IoT for a paper?

I need to know how to test the security of an implemented algorithm in the field of the IoT to state the test results in a paper. #IoT #Security #Blockchain #remix #Smart_Contract

01 July 2020 7,390 11 View

How to run Difference in Difference Different Time Periods?

Dear All, I have Panel Data fits difference in difference I regress the (Bilateral Investment Treaties-BIT) on (Bilateral FDI). BIT: is dummy taking 1 if BIT exists and Zero Otherwise....

29 May 2020 9,871 6 View

Thermodynamic properties of of the components of a certain solar thermophotovoltaic?

I am trying to simulate a solar TPV with a '1D Si/SiO2 PhC Emitter' and a single diode 'InGaAsSb pv cell'. now I am looking for 'thermal conductivity', 'Specific heat capacity' and 'density' of...

25 May 2020 7,818 3 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

To date, according to health authorities, 30% of the population's diseases are initiated by drinking water pollution. Do you know this problem?

The main studies are as follows: * regular and timely receipt of information about the state of the environment using automated systems (stationary and mobile); * operational analysis of the...

23 February 2021 912 5 View

Multinomial probabilities and confidence intervals - obtaining the CI of the reference category?

Hi, Some background to my question: I have fitted 1st order multinomial Markov models with covariates. Since all probabiilties (for each row of the transition matrix) sum to 1, I estimate only...

22 February 2021 2,541 4 View