I'm culturing C6/36 cells in DMEM for dengue virus.
There are two type of media in our lab, and I wonder which on to use.
Both of them are high glucose, but some compositions are different
1. DMEM high glucose, with 4500 mg/L D-glucose, with L-glutamine
2. DMEM high glucose, with 4500 mg/L D-glucose, with L-glutamine, 110 mg/L sodium pyruvate, with sodium bicarbonate
Which media is better to culture virus?
Is it critical when I use first media(without sodium pyruvate and sodium bicarbonate) to grow C6/36?