Poly-L-lysine is know for the seeding of cells but I just read an article relating the fact that cells could degrade Poly-L-lysine on coverslips during the culture day to get the cells to confluence. I saw that poly-D-lysine could be an alternative. Does someone already have experience with these two kinds of coating and can tell me which one is the best?
The purpose is to make staining of cells for microscopy imaging.
Hi, our lab using poly-D-lysine to coat chamber slide for cell culture. It work well.
It may depend on the cells you are using, but PDL is much more commonly used. I have used PDL for primary neuron culture. Probably the best strategy is to do test preparations with both coating types and see which one offers the best image for what you are trying to accomplish.
PDL works well, just make sure to rinse the coverslips to remove any residual PDS as cell do not like this.
Thanks a lot for your fast responses, i will then use poly-d-lysine to seed cells.
regards.
I have used Poly L lysine, just 30mins with 0.01% Poly L Lysine, wash with PBS and add cells. Works perfectley with HEK293.
I tried poly-l-lysine works fine with lymphocytes. Thanks for all your answers.
regards.
Liaque mohammed Latif iaque mohammed Latif could you please tell us the working concentration of PLL for coverslips coating? you mentioned 0.01% PLL. what is 100% PLL? I mean the the concentration of your stock PLL.
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