Quentin Duhault

3 Questions 5 Answers 0 Followers

Questions related from Quentin Duhault

Does anyone have a good protocol to coat a coverslip with antibodies ?

I would like to coat glass coverslips with antibodies in a sterile way does anyone have already tried ? If yes, what protocol did you use to make it possible. The coating would be made for a...

30 July 2014 6,441 3 View

Which kind of poly-lysine coating (L or D) is the best for fixing cells (adherent or non-adherent) ?

Poly-L-lysine is know for the seeding of cells but I just read an article relating the fact that cells could degrade Poly-L-lysine on coverslips during the culture day to get the cells to...

22 May 2014 5,588 8 View

Can anyone recommend a good immunofluorecence protocol for non adhesive cells in culture?

Which is the best way to adhere cells on slide/coverslips? I need to attach T cells on slide to make immunofluorecence assays and microscopy analysis

05 May 2014 6,077 4 View