Which is the relevant characterization technique to determine carrier separation ability and migration direction ?

22 April 2025 0 8K Report

Characterization technique to determine migration direction of monomer and composite

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Is there any way to determine the concentration of protein in a gel-ready protein sample?

I am performing western blot and recently i have been obtaining faint bands for the samples i had already run and had got darker bands. I wish to determine the concentration of protein in those...

08 May 2024 8,933 4 View

Hi researcher, I have a query regarding Web of Science. I have added a WoS indexed paper in WoS ResearcherID and it is showing not indexed?

So i have added a research paper in Web of Science Researcher ID and it is showing not indexed even though it is an Web of Science indexed journal. There are 2 papers i have published from the...

19 April 2024 7,643 1 View

Is there a readily available list of functional groups present in each phytochemical (alkaloids, flavanoids, resins, etc.)?

I have conducted phytochemical screening followed by FTIR for an aqueous plant extract and I want to know how to interpret the FTIR results to determine which phytochemicals are present based on...

02 April 2024 9,783 3 View

Hello all, can anyone help me with identifying which functional groups correspond to the peaks in the FTIR analysis below?

The FTIR analysis is of Mucuna Bracteata aqueous leaf extract. I referred to a few articles to understand which functional group corresponds to which wavenumber but I encountered ambiguities. Any...

02 April 2024 1,017 3 View

In SCLC conduction mechanism, what is the interpretation whose slope value is greater than 2?

it is known SCLC conduction mechanism has three regions based on bias voltage. At higher voltage after child's law is obeyed(I directly proportional to V^2). further increase in voltage increases...

19 March 2024 5,493 0 View

Which among the membrane filter paper can be used for the immobilization of Ag/TiO2 nanocomposite?

Technique to develop immobilization of nanocomposite using membrane filter paper

15 March 2024 9,380 0 View

Have iron oxide nanoparticles ever been used as a solid acid catalyst?

Is there literature available on this where iron oxide nanocatalysts are substituted for general iron oxide catalysts.

10 March 2024 5,397 1 View

`which is the best immobilization media for silver titanium based photocatalysts that can be used for the degradation of antibiotics?

Techniques used for immobilization of Ag-TiO2 catalysts

09 March 2024 7,671 1 View

Which website/writing software is preferred for writing a research article for publication?

My apologies if this is a silly question but I am a beginner/student and an amateur who is making his first attempt to write a manuscript. My mentor has been disappointingly unhelpful in this...

02 March 2024 233 4 View

What is the standard protocol for microbial growth media development from biomass hydrolysate?

The microbe is yeast (Saccharomyces cerevisiae) and acid hydrolysis is the route of treatment. A good paper highlighting all the necessary steps and sequences would be highly appreciated since...

29 February 2024 3,412 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Is it possible to manufacture polypropylene sheets using a biaxial process?

I know that films and other low-thickness laminated products are produced using biaxial orientation, but is there something similar for sheets with a thickness of around 6 mm? Or are they...

21 July 2024 1,559 1 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized?

What are the opportunities and limitations for using digestate ion different crops and soil typs and how can its application be optimized - I have few of them listed and can be updated in this...

13 July 2024 2,431 2 View

Why am I seeing only one initial maximum in torque-time curves for the fusion of PVC in a Brabender?

We're studying the stability and fusion behavior of multiple PVC compounds in a Brabender. After a few trials, we set the temperature at 195 °C and the rotor speed at 70 rpm. Samples differ in...

12 July 2024 2,768 1 View

Can I use BSA for a Histological Blocking Solution?

Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...

07 July 2024 7,967 3 View

How long can I store mouse tissues in neutral buffered formalin for, and how often should I add fresh formalin?

My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...

06 July 2024 8,360 2 View